Isolation of microsatellites from unknown genomes using known genomes as enrichment templates
نویسندگان
چکیده
منابع مشابه
Evolution of Coding Microsatellites in Primate Genomes
Microsatellites (SSRs) are highly susceptible to expansions and contractions. When located in a coding sequence, the insertion or the deletion of a single unit for a mono-, di-, tetra-, or penta(nucleotide)-SSR creates a frameshift. As a consequence, one would expect to find only very few of these SSRs in coding sequences because of their strong deleterious potential. Unexpectedly, genomes cont...
متن کاملDeep Subsurface Life from North Pond: Enrichment, Isolation, Characterization and Genomes of Heterotrophic Bacteria
Studies of subsurface microorganisms have yielded few environmentally relevant isolates for laboratory studies. In order to address this lack of cultivated microorganisms, we initiated several enrichments on sediment and underlying basalt samples from North Pond, a sediment basin ringed by basalt outcrops underlying an oligotrophic water-column west of the Mid-Atlantic Ridge at 22°N. In contras...
متن کاملanalysis of simple and imperfect microsatellites in ebolavirus species and other genomes of filoviridae family
background microsatellites have evoked the interest of researchers owing to their applications in different fields such as dna fingerprinting, genetic mapping, population genetics, forensics, paternity studies and evolution. objectives the present study focused on the analysis of simple sequence repeats (ssrs) in genomes of seven species from three genera of the filoviridae family. materials an...
متن کاملSurvey of compound microsatellites in multiple Lactobacillus genomes.
Distinct simple sequence repeats with 2 or more individual microsatellites joined together or lying adjacent to each other are identified as compound microsatellites. Investigation of such composite microsatellites in the genomes of genus Lactobacillus was the aim of this study. In silico inspection of microsatellite clustering in genomes of 14 Lactobacillus species revealed a wealth of compoun...
متن کاملEvaluation of the Number of Different Genomes on Medium and Identification of Known Genomes Using Composition Spectra Approach
The article presents the theoretical foundations of the algorithm for calculating the number of different genomes in the medium under study and of two algorithms for determining the presence of a particular (known) genome in this medium. The approach is based on the analysis of the compositional spectra of subsequently sequenced samples of the medium. The theoretical estimations required for th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Limnology and Oceanography: Methods
سال: 2008
ISSN: 1541-5856
DOI: 10.4319/lom.2008.6.412